![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K1211002:Design
Designed by: Joel Sher Group: iGEM13_Yale (2013-08-16)
Pseudomonas resinovorans Polyhydroxyalkanoate synthase
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1411
Illegal PstI site found at 25
Illegal PstI site found at 118
Illegal PstI site found at 208
Illegal PstI site found at 280
Illegal PstI site found at 742 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1411
Illegal PstI site found at 25
Illegal PstI site found at 118
Illegal PstI site found at 208
Illegal PstI site found at 280
Illegal PstI site found at 742 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1411
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1411
Illegal PstI site found at 25
Illegal PstI site found at 118
Illegal PstI site found at 208
Illegal PstI site found at 280
Illegal PstI site found at 742 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1411
Illegal PstI site found at 25
Illegal PstI site found at 118
Illegal PstI site found at 208
Illegal PstI site found at 280
Illegal PstI site found at 742
Illegal NgoMIV site found at 1167
Illegal NgoMIV site found at 1212
Illegal AgeI site found at 448
Illegal AgeI site found at 853
Illegal AgeI site found at 1486 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 251
Design Notes
The sequence was codon optimized and four amino acid mutations were introduced. Also, mutations were introduced in order to remove restriction sites.
Source
GenBank